ACC Deaminase from Issatchenkia occidentalis

Differentiation of 1-aminocyclopropane-1-carboxylate (ACC) deaminase from its homologs is the key for identifying bacteria containing ACC deaminase.

1-Aminocyclopropane-1-carboxylate (ACC) deaminase-mediated reduction of ethylene generation in plants under abiotic stresses is a key mechanism by which bacteria can promote plant growth. Misidentification of ACC deaminase and the ACC deaminase structure gene (acdS) can lead to overestimation of the number of bacteria containing ACC deaminase and their function in ecosystems. Previous non-speci...

Measurement of ACC-Deaminase Production in Halophilic, Alkalophilic and Haloalkalophilic Bacterial Isolates in Soil

Strains with 1-aminocyclopropane-1-carboxylate (ACC) deaminase activity can cause plant growth on stress condition. In the presence of bacteria with the ability of ACC deaminase production in the rhizosphere, it is possible to convert the ethylene precursor (ACC) to α-ketobutyrate  and ammonium, reducing the ethylene levels in host plants. In order to measure the power and level of A...

Reflections from ACC 2015

Purification and properties of glutathione transferase from Issatchenkia orientalis.

Glutathione transferase (GST) (EC 2.5.1.18) was purified from a cell extract of Issatchenkia orientalis, and two GST isoenzymes were isolated. They had molecular weights of 37,500 and 40,000 and were designated GST Y-1 and GST Y-2, respectively. GST Y-1 and GST Y-2 gave single bands with molecular weights of 22,000 and 23,500, respectively, on sodium dodecyl sulfate-polyacrylamide gel electroph...

Characterization of Accdeaminase in Plant 361 Characterization of Acc Deaminase in Plant Growth Promoting Pseudomonas from Tannery Sludge

The plant growth promoting strains isolated from tannery sludge and charaeteri/ed is Pseudomonas strain. The plant growth promoting Pseudomonas pittidu can utilize 1-aniinocyclopropanc-l-carboxylase (ACC) as a sole nitrogen source because it possess the unusual enzyme ACC dcaminase, which hydroly/es ACC to ammonia and a-ketobutyrate. This enzyme which is though to be intimately involved in the ...